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载入果蝇与神经末梢钙指标
JoVE Journal
Biology
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JoVE Journal
Biology
Loading Drosophila Nerve Terminals with Calcium Indicators
Please note that all translations are automatically generated.
Click here for the English version.
载入果蝇与神经末梢钙指标
DOI:
10.3791/250-v
•
15:16 min
•
July 30, 2007
•
Adam J. Rossano
,
Gregory T. Macleod
1
Department of Physiology
,
University of Texas Health Science Center at San Antonio (UTHSCSA)
Chapters
00:03
Introduction
01:08
Dissection of larva
05:37
Nerve loading apparatus
07:53
Loading the nerve
10:32
Considerations for successful loading
11:30
Dye removal and incubation
12:29
Preparing for imaging
13:57
Live imaging
Summary
Automatic Translation
English (Original)
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Automatic Translation
钙是一种在神经系统中无处不在的使者,触发神经递质释放和突触强度的变化。在这里,我们展示了
一个
加载的
Ca
2 +指标到果蝇的神经终端的技术。我们也证明所需设备的制造,并强调技术的成功的关键点。
Tags
Drosophila
Nerve Terminals
Calcium Indicators
Neurotransmitter Release
Synaptic Plasticity
Learning And Memory
Genetically Malleable
Physiologically Accessible
Genetically-encoded Fluorescent Indicators
Ca2+ Changes
Synthetic Fluorescent Indicators
Dextran-conjugated Synthetic Ca2+ Indicators
Live Nerve Terminals
Drosophila Larvae
Static Electricity Discharges
Extended Loading Periods
Axon Survival
Low Affinity Dextran-conjugated Ca2+-indicators
Fluo-4
Rhod
Article
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