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Journal
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Immunology and Infection
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人外周血NK细胞的扩展,纯化和功能评估
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
Please note that all translations are automatically generated.
Click here for the English version.
人外周血NK细胞的扩展,纯化和功能评估
DOI:
10.3791/2540-v
•
10:44 min
•
February 02, 2011
•
Srinivas S. Somanchi
,
Vladimir V. Senyukov
,
Cecele J. Denman
,
Dean A. Lee
1
Division of Pediatrics
,
MD Anderson Cancer Center – University of Texas
Chapters
00:05
Title
01:13
Introduction
01:40
Isolation of Cell Fractions from Buffy Coat
03:34
NK Cell Expansion
06:34
NK Cell Cytotoxicity Assay
08:33
Representative Results
09:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一种方法,有效地扩大和净化人类NK细胞的大量和评估它们的功能。
Tags
Expansion
Purification
Functional Assessment
Human
Peripheral Blood
NK Cells
Natural Killer Cells
Immune Surveillance
Infectious Microorganisms
Tumors
Limited Availability
In Vitro
NK Cell Immunotherapy
Method
Efficiently Expand
Vast Quantities
Functional NK Cells
Ex Vivo
K562 Cells Expressing Membrane-bound IL21
Artificial Antigen-presenting Cell (aAPC)
Cell Product
Steady-state Leukapheresis
Donor
Depletion Of T Cells
Positive Selection Of NK Cells
IL-2 Overnight Activation
Small Numbers Of NK Cells Delivered In Clinical Trials
Inability To Propagate NK Cells In Vitro
Generating Sufficient Cell Numbers
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