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Chapters
- 00:05Title
- 01:12Extract Epithelial Stem Cells from Human Skin
- 03:11Culture Primary Epithelial Stem Cells
- 05:19Immortalize Epithelial Stem Cells
- 06:52Skin Epithelial Stem Cell Culture and Differentiation Results
- 08:02Conclusion
Un modo rapido, robusto di isolare vitali cellule staminali epiteliali adulte di pelle umana è descritta. Il metodo utilizza la digestione enzimatica della matrice di collagene della pelle, seguita da spiumatura dei follicoli piliferi e l'isolamento delle sospensioni singola cella o frammenti di tessuto per la coltura cellulare.
Tags
IsolationCultureAdult Epithelial Stem CellsHuman SkinHomeostasisAsymmetric DivisionsDaughter CellsStem Cell PhenotypeTransit-amplifying CellsStem Cell NicheRapid ProliferationTerminal DifferentiationEpidermisHair FollicleIntestineIn Vitro Proliferative PotentialLabel-retaining CellsMulti-potent Stem CellsRodent Hair Follicle Stem CellsEpidermisSebaceous GlandsHair FolliclesHuman Hair Follicle Bulge RegionMulti-potentialityBiomedical ResearchDevelopmental BiologyDifferentiationTumorigenesisTherapeutic Utility
