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克隆形成含量:贴壁细胞
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Biology
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Biology
Clonogenic Assay: Adherent Cells
Please note that all translations are automatically generated.
Click here for the English version.
克隆形成含量:贴壁细胞
DOI:
10.3791/2573-v
•
05:30 min
•
March 13, 2011
•
Haloom Rafehi
2
,
Christian Orlowski
2,3
,
George T. Georgiadis
,
Katherine Ververis
4
,
Assam El-Osta
3
,
Tom C. Karagiannis
2
1
Epigenomic Medicine, BakerIDI Heart and Diabetes Institute
,
The Alfred Medical Research and Education Precinct
,
2
Department of Pathology
,
The University of Melbourne
,
3
Epigenetics in Human Health and Disease, BakerIDI Heart and Diabetes Institute
,
The Alfred Medical Research and Education Precinct
,
4
Department of Anatomy and Cellular Biology
,
The University of Melbourne
Chapters
00:01
Title
00:05
Introduction
01:11
Cell Preparation
01:44
Irradiation
03:30
Plating
03:51
Fixing and Staining
05:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
克隆形成生殖可行性的评估检测的适用性已成立超过50年。在这里,我们展示了贴壁细胞进行克隆形成实验的一般程序。
Tags
Clonogenic Assay
Colony Forming Assay
Reproductive Viability
Radiation-dose Response Curve
X-ray Irradiated Cells
Ionising Radiation
Cytotoxic Agents
Genetic Manipulation
Radiation Biology
Radiation Sensitivity
Radiation Modifying Compounds
Cytotoxic Agents
Anti-cancer Therapeutics
Adherent Cells
Cell Monolayer
Single Cell Suspensions
Petri Dishes
Fixing And Staining Colonies
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