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确定远距离的DNA相互作用的相关染色体陷阱
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Associated Chromosome Trap for Identifying Long-range DNA Interactions
Please note that all translations are automatically generated.
Click here for the English version.
确定远距离的DNA相互作用的相关染色体陷阱
DOI:
10.3791/2621-v
•
14:49 min
•
April 23, 2011
•
Jianqun Ling
,
Andrew R. Hoffman
1
Medical Service, VA Palo Alto Health Care System
,
Stanford University School of Medicine
Chapters
00:05
Title
01:30
Formaldehyde Fixation of Long-Range Chromatin Interactions and Nuclei Isolation
03:36
Digestion, Ligation and Purification of Interaction DNA Segments
06:10
Digestion with Msp I and Ligation with Oligonucleotide Linkers
07:50
PCR Amplification and Sequence Analysis
11:06
Act Assay Representative Results
14:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
相关的染色体陷阱(ACT)的分析是用于识别远射DNA相互作用的一种新的不带偏见的方法。远距离DNA相互作用的特性,将使我们在正常的生理和患病状态,以确定核架构的关系,基因的表达。
Tags
Associated Chromosome Trap
Long-range DNA Interactions
Chromatin Structure
Chromosome Territories
Gene Expression
Transcriptional Factories
Co-localization
ACT Assay
Chromosome Conformation Capture Technique
Transcriptional Regulation
Nuclear Architecture
Gene Expression In Disease States
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