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传代人类神经干细胞
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JoVE Journal
Biology
Passaging Human Neural Stem Cells
Please note that all translations are automatically generated.
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传代人类神经干细胞
DOI:
10.3791/263-v
•
10:16 min
•
August 22, 2007
•
Steven Marchenko
,
Lisa Flanagan
1
Department of Pathology
,
University of California, Irvine (UCI)
Chapters
00:02
Title
00:07
Introduction
01:42
Preparing the Coated Flask and Dissociating the Cells
06:14
Centrifuging and Resuspending Cells
09:44
Counting Cells in Suspension
Summary
Automatic Translation
English (Original)
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Automatic Translation
操作能力在体外培养人类神经干/前体细胞(hNSPCs)允许调查作为细胞移植用于治疗目的的实用价值与探索人类的神经系统发育。该协议提出希望增加人类干细胞研究的可重复性的培养和传代hNSPCs的方法。
Tags
Passaging
Human Neural Stem Cells
HNSPCs
In Vitro
Cell Transplants
Therapeutic Purposes
Human Neural Development
Pathology
Culturing
Standardizing Technique
Reproducibility
Stem Cell Research
Cadaveric Postnatal Brain Cortices
National Human Neural Stem Cell Resource
Adherent Cultures
Fibronectin Coating
DMEM:F12 Serum-free Media
EGF
FGF
PDGF
Bipolar Morphology
Undifferentiated Neural Stem Cells
Nestin
Sox2
Article
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