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Biology
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双分子荧光互补
JoVE Journal
Biology
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JoVE Journal
Biology
Bimolecular Fluorescence Complementation
Please note that all translations are automatically generated.
Click here for the English version.
双分子荧光互补
DOI:
10.3791/2643-v
•
08:54 min
•
April 15, 2011
•
Katy A. Wong
,
John P. O’Bryan
1
Department of Pharmacology
,
University of Illinois at Chicago
Chapters
00:05
Title
01:06
Introduction
01:51
BiFC Calibration
03:04
Plating and Transfection of Cells
04:37
Imaging Cells
07:08
Monitoring SH3 Interactions of ITSN
08:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在确定的时空调控细胞信号蛋白质的亚细胞定位是很重要的。在这里,我们描述了一个简单的方法监测在细胞内的蛋白质的空间相互作用的双分子荧光互补(附设)。
Tags
Bimolecular Fluorescence Complementation
Subcellular Distribution
Signaling Complexes
Immunoprecipitation
Spatial Localization
BiFC
Protein Complexes
Fluororescent Protein
Amino-terminal
Carboxy-terminal
Protein Fusion
Interaction
Reconstitution
Fluorophore
Limitation
Transient Interactions
Weak Interactions
Kinetic Analysis
Complex Dynamics
Flourophore Maturation
Real Time Interactions
Protein Fragment Complementation Assay
Reporter Proteins
Green Fluorescent Protein Variants
Dihydrofolate Reductase
B-lactamase
Luciferase
Protein:protein Interactions
Fluorescence Co-localization
Förster Resonance Energy Transfer (FRET)
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