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高通量测量和环境样品中的有机磷分类
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
High-Throughput Measurement and Classification of Organic P in Environmental Samples
DOI:

08:58 min

June 08, 2011

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Chapters

  • 00:05Title
  • 01:27Phosphorus Extraction and pH Adjustment
  • 02:13Enzyme Stock Preparation
  • 02:59P Calibration Curve and Controls
  • 04:23Sample + Enzyme Incubation
  • 05:20Colorimetric Measurement of Released and Background Inorganic P
  • 06:09Classification of P Compounds
  • 07:35HTP Analysis of Phosphorus in Soil
  • 08:26Conclusion

Summary

Automatic Translation

此协议,介绍了一种酶水解,利用酶标仪来衡量和分类,土壤磷P单酯,磷酯和无机体育多达96个样品,可以在一次在一个标准的实验室测量高通量的方法。

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