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Immunology and Infection
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制备和使用艾滋病毒感染主的CD4 + T细胞作为靶细胞,自然杀伤细胞的细胞毒性检测
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
Please note that all translations are automatically generated.
Click here for the English version.
制备和使用艾滋病毒感染主的CD4 + T细胞作为靶细胞,自然杀伤细胞的细胞毒性检测
DOI:
10.3791/2668-v
•
12:07 min
•
March 14, 2011
•
Zachary B. Davis
,
Jeffrey P. Ward
,
Edward Barker
1
Department of Immunology and Microbiology
,
Rush University Medical Center
Chapters
00:05
Title
01:14
Infection of CD4+ T-Cells with HIV-1
02:42
Purification of HIV-1 Infected Cells
03:41
CD107a Degranulation Assay
06:41
51Cr Release Assay
10:07
Evaluation of CD107a Degranulation and NK Cell Cytotoxicity
11:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
细胞毒性实验来衡量HIV感染的细胞自然杀伤细胞裂解反应是有限的靶细胞的纯度。我们这里展示的高度纯化的HIV - 1感染的主要T细胞爆炸的人口利用HIV - 1病毒的能力,向下调整的CD4隔离。
Tags
HIV-1
Primary CD4+ T-cells
Natural Killer Cells
Cytotoxic Assays
Target Cells
NK Cell Function
Cytolytic Activity
HIV-infected Cells
T-cell Blasts
Infected-T-cell Lines
Autologous Primary Cells
Major Histocompatibility Complex Class I Mismatches
NK Cell Lysis
Infected Cell To Uninfected Cell Ratio
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