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Bioengineering
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3D楕円体の生成のための簡単なハンギングドロップ細胞培養プロトコル
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids
Please note that all translations are automatically generated.
Click here for the English version.
3D楕円体の生成のための簡単なハンギングドロップ細胞培養プロトコル
DOI:
10.3791/2720-v
•
07:24 min
•
May 06, 2011
•
Ramsey Foty
1
Department of Surgery
,
UMDNJ-Robert Wood Johnson Medical School
Chapters
00:05
Title
01:14
Preparing a Single Cell Suspension
02:57
Hanging Drop Formation
04:38
Representative Results of Hanging Drop Cultures, Compaction of Cellular Aggregates and Spheroid Bodies
07:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は、細胞 - 細胞相互作用の違いを定量化するために三次元組織のような回転楕円体とそれらの潜在的なアプリケーションを生成する単純な、急速な方法を説明します。
Tags
Hanging Drop Cell Culture
3D Spheroids
Cell-cell Cohesion
Cell-substratum Adhesion
Monolayer Culture
Rigid Substrates
Intimate Connections
Extracellular Matrix Components
Chemical Milieu
Physical Forces
Cellular Morphology
Signaling
Collagen Gels
Biomaterial Scaffolds
Direct Cell-cell Adhesion Architecture
Culture Systems
ECM Products
Physiological Conditions
Biological Agent
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