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Immunology and Infection
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β细胞死亡介导细胞毒性T淋巴细胞的方法来评估
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
Please note that all translations are automatically generated.
Click here for the English version.
β细胞死亡介导细胞毒性T淋巴细胞的方法来评估
DOI:
10.3791/2724-v
•
12:12 min
•
June 16, 2011
•
Jing Chen
,
Scott Grieshaber
,
Clayton E. Mathews
1
Departments of Pathology, Immunology and Laboratory Medicine, College of Medicine
,
University of Florida
Chapters
00:05
Title
01:38
Introduction
02:29
Cell Culture
04:42
Cell-mediated Lymphocytotoxicity (CML Assay)
07:29
Live Cell Imaging to Asses CTL-mediated Beta Cell Death
10:10
Representative Cell Death Assay Data
11:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
细胞介导的淋巴细胞毒(CML)的检测,可用于测试自身反应性的反应,并在体外研究细胞死亡的机制。然而,利用活细胞共聚焦显微成像技术与荧光染料,类型和细胞死亡以及途径利用动力学可以更详细的研究。
Tags
Beta Cell Death
Cytotoxic T Lymphocytes
Type 1 Diabetes
Insulin Production
Mechanisms Of Beta Cell Death
Cell-mediated Lymphocytotoxicity Assay
51Cr Release
Effector Cells
Autoreactive CD8+ Cytotoxic T Lymphocytes
AI4 T Cell Receptor
Specific Lysis
Mitochondria
Mitochondrial Functional Changes
Mitochondrial Membrane Potential Dye
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