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无菌实验室技术:电镀方法
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Aseptic Laboratory Techniques: Plating Methods
Please note that all translations are automatically generated.
Click here for the English version.
无菌实验室技术:电镀方法
DOI:
10.3791/3064-v
•
18:00 min
•
May 11, 2012
•
Erin R. Sanders
1
Microbiology, Immunology, and Molecular Genetics
,
University of California, Los Angeles
Chapters
00:05
Title
01:51
A Safe and Sterile Workplace
03:04
Streak Plate Procedure: Isolation of Bacterial Colonies using the Quadrant Method
05:26
Pour Plate Procedure: Enumeration of Bacterial Cells in a Mixed Sample
06:38
Spread-plating: Turntable with Glass or Metal Rod
07:59
Spread-plating: the Copacabana Method
09:11
Soft Agar Overlay Procedure: Plaque Assay
11:52
Replica Plate Protocol: Screening Mutants and Auxotrophs
13:59
Clean Up
14:25
Practical Applications
17:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
媒体和试剂用于培养微生物的工作时,必须实行无菌技术,以确保污染降到最低。多种电镀方法通常用于隔离,传播,或列举细菌和噬菌体,所有这些都纳入程序,维护实验材料的无菌。
Tags
Aseptic Laboratory Techniques
Plating Methods
Microorganisms
Contamination
Sterilize Work Surfaces
Prevent Contact
Sterile Instruments
Sterile Solutions
Plating Methods
Isolate Microorganisms
Bacteria
Phage
Aseptic Technique
Streak-plating
Pour-plating
Spread-plating
Soft Agar Overlays
Isolate Phage
Enumerate Plaques
Replica-plating
Biosafety Level 1 (BSL-1)
Biosafety Level 2 (BSL-2)
Biosafety Cabinet
Biohazard Classification
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