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人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具

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Immunology and Infection

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JoVE Journal Immunology and Infection

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

Please note that all translations are automatically generated. Click here for the English version.

人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具

DOI:

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14:01 min

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July 22, 2011

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Jill Waukau, Jeffrey Woodliff, Sanja Glisic

¹Department of Pediatrics/Allergy,Medical College of Wisconsin , ²Flow Cytometry Core Facility,Medical College of Wisconsin , ³Max McGee National Research Center for Juvenile Diabetes and Human Molecular Genetics Center,Medical College of Wisconsin

Chapters

00:05Title
02:08Tosylactivated Bead Coating
04:25PBMC Isolation
05:41MACS Pre-sort of CD4 T Cells
08:05Fluorescent Activated Cell Sorting (FACS) Isolation
09:59Cell Culture Setup, Harvesting, and Counting
11:32Tregs Differ in their Ability to Successfully Suppress Naive and in vivo-activated T Cells
13:34Conclusion

Summary

Automatic Translation

调节性T细胞是免疫系统的强有力的抑制器。有一个独特的表面标志物来定义它们的缺乏，因此，调节性T细胞的定义主要功能。在这里，我们描述了一个优化的<em>在体外</em>检测能够识别风险科目的免疫失衡发展T1D。

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