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Chapters
- 00:05Title
- 01:47Preparation of Silicone Membrane Dishes and Adherence of Cells
- 03:30Substrate Strain Experiments
- 06:50Substrate Strain Analysis
- 08:35Representative Results for Substrate Strain Application on the Interphase Cell Nucleus
- 09:57Microneedle Manipulation Experiment
- 12:19Microneedle Manipulation Analysis
- 13:50Representative Results for Microneedle Manipulation of the Interphase Cell Nucleus
- 15:58Conclusion
Vi presenterar två oberoende, mikroskop-baserade verktyg för att mäta den inducerade kärnkraft och cytoskelettala deformationer i singel, lever fastsittande celler som svar på globala eller lokala stam ansökan. Dessa tekniker används för att bestämma nukleära stelhet (dvs deformerbarhet) och sond intracellulära kraftöverföring mellan kärnan och cytoskelettet.
Tags
Biophysical AssaysMechanical PropertiesInterphase Cell NucleusSubstrate Strain ApplicationMicroneedle ManipulationEukaryotic CellsNucleus StiffnessCytoskeletonBiomechanical BehaviorPhysiological ConditionsPathological ConditionsMigrating NeutrophilsInvading Cancer CellsExtravasationNarrow SpacesMuscleStructural SupportRepetitive Mechanical StressCellular ArchitectureNuclear Envelope ProteinsLaminsNesprinsNucleo-cytoskeletal CouplingHuman Diseases
