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Chapters
- 00:05Title
- 01:47Preparation of Silicone Membrane Dishes and Adherence of Cells
- 03:30Substrate Strain Experiments
- 06:50Substrate Strain Analysis
- 08:35Representative Results for Substrate Strain Application on the Interphase Cell Nucleus
- 09:57Microneedle Manipulation Experiment
- 12:19Microneedle Manipulation Analysis
- 13:50Representative Results for Microneedle Manipulation of the Interphase Cell Nucleus
- 15:58Conclusion
Se presentan dos independientes, basados en microscopio de herramientas para medir las deformaciones inducidas nuclear y del citoesqueleto en habitaciones individuales, que viven las células adherentes en respuesta a la aplicación de la tensión global o localizada. Estas técnicas se utilizan para determinar la rigidez nuclear (es decir, la deformabilidad) y para investigar la transmisión de fuerza intracelular entre el núcleo y el citoesqueleto.
Tags
Biophysical AssaysMechanical PropertiesInterphase Cell NucleusSubstrate Strain ApplicationMicroneedle ManipulationEukaryotic CellsNucleus StiffnessCytoskeletonBiomechanical BehaviorPhysiological ConditionsPathological ConditionsMigrating NeutrophilsInvading Cancer CellsExtravasationNarrow SpacesMuscleStructural SupportRepetitive Mechanical StressCellular ArchitectureNuclear Envelope ProteinsLaminsNesprinsNucleo-cytoskeletal CouplingHuman Diseases
