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Journal
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Biology
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生物物理检测探头相间细胞核的力学性能:基材应变的应用和微针操纵
JoVE Journal
Biology
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JoVE Journal
Biology
Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
Please note that all translations are automatically generated.
Click here for the English version.
生物物理检测探头相间细胞核的力学性能:基材应变的应用和微针操纵
DOI:
10.3791/3087-v
•
16:27 min
•
September 14, 2011
•
Maria L. Lombardi
,
Monika Zwerger
,
Jan Lammerding
2
1
Brigham and Women’s Hospital / Harvard Medical School
,
Department of Medicine, Cardiovascular Division
,
2
Weill Institute for Cell and Molecular Biology & Department of Biomedical Engineering
,
Cornell University
Chapters
00:05
Title
01:47
Preparation of Silicone Membrane Dishes and Adherence of Cells
03:30
Substrate Strain Experiments
06:50
Substrate Strain Analysis
08:35
Representative Results for Substrate Strain Application on the Interphase Cell Nucleus
09:57
Microneedle Manipulation Experiment
12:19
Microneedle Manipulation Analysis
13:50
Representative Results for Microneedle Manipulation of the Interphase Cell Nucleus
15:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们现在两个独立的显微镜为基础的工具来衡量,在应对全球性或局部应变的应用单一,生活贴壁细胞诱导核和细胞骨架变形。这些技术被用于确定核刚度(即变形)和探测细胞内的细胞核和细胞骨架的之间的传力。
Tags
Biophysical Assays
Mechanical Properties
Interphase Cell Nucleus
Substrate Strain Application
Microneedle Manipulation
Eukaryotic Cells
Nucleus Stiffness
Cytoskeleton
Biomechanical Behavior
Physiological Conditions
Pathological Conditions
Migrating Neutrophils
Invading Cancer Cells
Extravasation
Narrow Spaces
Muscle
Structural Support
Repetitive Mechanical Stress
Cellular Architecture
Nuclear Envelope Proteins
Lamins
Nesprins
Nucleo-cytoskeletal Coupling
Human Diseases
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