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Journal
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Immunology and Infection
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分离单链DNA,双链DNA和RNA病毒使用羟基磷灰石色谱从环境社区
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
Please note that all translations are automatically generated.
Click here for the English version.
分离单链DNA,双链DNA和RNA病毒使用羟基磷灰石色谱从环境社区
DOI:
10.3791/3146-v
•
13:46 min
•
September 29, 2011
•
Douglas W. Fadrosh
,
Cynthia Andrews-Pfannkoch
,
Shannon J. Williamson
1
Department of Microbial and Environmental Genomics
,
The J. Craig Venter Institute
,
2
Department of Synthetic Biology and Bioenergy
,
The J. Craig Venter Institute
Chapters
00:05
Title
02:35
Preparation of Solutions
03:15
Preparation of Econo-Column
05:34
Hydroxyapatite Chromatography
09:16
Desalting Nucleic Acid Samples
11:03
Examples of Separation of Single-stranded DNA, Double-stranded DNA and RNA
13:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了独立于环境病毒社区的单链DNA,双链DNA和RNA分子的有效方法。核酸是分次使用含有磷酸盐缓冲液浓度的增加羟基磷灰石色谱。这种方法允许所有病毒从环境样品中的核酸类型的隔离。
Tags
Single-stranded DNA
Double-stranded DNA
RNA
Environmental Viral Community
Hydroxyapatite Chromatography
Bacteriophages
Genetic Diversity
Metagenomic Techniques
Taxonomic Diversity
Functional Potential
Library Construction Protocols
Nuclease Treatment
Collective Gene Complement
Virus Diversity
Purified Nucleic Acid Subtypes
Calcium Phosphate
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