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定量分析和HO -核酸内切酶的形成，刺激单链退火染色体易位酿酒酵母</em
JoVE Journal
Biology
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JoVE Journal Biology
Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
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定量分析和HO -核酸内切酶的形成，刺激单链退火染色体易位酿酒酵母</em

DOI:
10.3791/3150-v

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09:40 min

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September 23, 2011

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, , ,
1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology,City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology,University of Southern California, Norris Comprehensive Cancer Center

Chapters

  • 00:05Title
  • 01:16Determining HO-stimulated Translocation Frequencies
  • 02:40Determining Plating Efficiencies
  • 04:05Southern Blot Analysis
  • 04:57Chromosome Blot Analysis
  • 06:13Quantitation and Analysis of Chromosomal Translocations
  • 09:21Conclusion

Summary

Automatic Translation

Automatic Translation

何刺激的易位检测监控以下二倍体多个位点的DNA双链断裂创造单链退火<em>酿酒酵母</em>。这种机制可能模式在高等真核生物体细胞暴露于高剂量的电离辐射的基因组重排。

Tags

QuantitationAnalysisFormationHO-EndonucleaseChromosomal TranslocationsSingle-Strand AnnealingSaccharomyces CerevisiaeGenetic VariationGenomic RearrangementsRepetitive ElementsEukaryotic GenomeRetrotransposon OriginLINEsSINEsExchange EventsGenome RearrangementsTranslocationsGene DosageGene ExpressionAutoimmune DiseasesCardiovascular DiseasesCancerHomologous RecombinationNon-homologous End Joining (NHEJ)Double-strand Breaks (DSBs)Mitotic CellsDNA ReplicationOxidative LesionsIonizing Radiation (IR)Exogenous DNA Damaging Agents

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