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Quantifizierung und Analyse der Bildung von HO-Endonuklease Stimulated chromosomalen Translokationen von Single-Strand Annealing in Saccharomyces cerevisiae</em
JoVE Journal
Biology
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JoVE Journal Biology
Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
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Quantifizierung und Analyse der Bildung von HO-Endonuklease Stimulated chromosomalen Translokationen von Single-Strand Annealing in Saccharomyces cerevisiae</em

DOI:
10.3791/3150-v

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09:40 min

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September 23, 2011

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, , ,
1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology,City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology,University of Southern California, Norris Comprehensive Cancer Center

Chapters

  • 00:05Title
  • 01:16Determining HO-stimulated Translocation Frequencies
  • 02:40Determining Plating Efficiencies
  • 04:05Southern Blot Analysis
  • 04:57Chromosome Blot Analysis
  • 06:13Quantitation and Analysis of Chromosomal Translocations
  • 09:21Conclusion

Summary

Automatic Translation

Automatic Translation

Die HO-stimulierte Translokation Assay überwacht Einzelstrang-Glühen nach der Gründung von DNA-Doppelstrangbrüche an mehreren Loci in diploiden<em> Saccharomyces cerevisiae</em>. Dieser Mechanismus kann Genom Umlagerungen in somatischen Zellen höherer Eukaryonten nach Exposition mit hohen Dosen ionisierender Strahlung Modell.

Tags

QuantitationAnalysisFormationHO-EndonucleaseChromosomal TranslocationsSingle-Strand AnnealingSaccharomyces CerevisiaeGenetic VariationGenomic RearrangementsRepetitive ElementsEukaryotic GenomeRetrotransposon OriginLINEsSINEsExchange EventsGenome RearrangementsTranslocationsGene DosageGene ExpressionAutoimmune DiseasesCardiovascular DiseasesCancerHomologous RecombinationNon-homologous End Joining (NHEJ)Double-strand Breaks (DSBs)Mitotic CellsDNA ReplicationOxidative LesionsIonizing Radiation (IR)Exogenous DNA Damaging Agents

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