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定量分析和HO -核酸内切酶的形成,刺激单链退火染色体易位
酿酒酵母</em
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in
Saccharomyces cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
定量分析和HO -核酸内切酶的形成,刺激单链退火染色体易位
酿酒酵母</em
DOI:
10.3791/3150-v
•
09:40 min
•
September 23, 2011
•
Lauren Liddell
2
,
Glenn Manthey
,
Nicholas Pannunzio
,
Adam Bailis
1
Irell & Manella Graduate School of Biological Sciences
,
2
Department of Molecular and Cellular Biology
,
City of Hope Comprehensive Cancer Center and Beckman Research Institute
,
3
Department of Biochemistry and Molecular Biology
,
University of Southern California, Norris Comprehensive Cancer Center
Chapters
00:05
Title
01:16
Determining HO-stimulated Translocation Frequencies
02:40
Determining Plating Efficiencies
04:05
Southern Blot Analysis
04:57
Chromosome Blot Analysis
06:13
Quantitation and Analysis of Chromosomal Translocations
09:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
何刺激的易位检测监控以下二倍体多个位点的DNA双链断裂创造单链退火<em>酿酒酵母</em>。这种机制可能模式在高等真核生物体细胞暴露于高剂量的电离辐射的基因组重排。
Tags
Quantitation
Analysis
Formation
HO-Endonuclease
Chromosomal Translocations
Single-Strand Annealing
Saccharomyces Cerevisiae
Genetic Variation
Genomic Rearrangements
Repetitive Elements
Eukaryotic Genome
Retrotransposon Origin
LINEs
SINEs
Exchange Events
Genome Rearrangements
Translocations
Gene Dosage
Gene Expression
Autoimmune Diseases
Cardiovascular Diseases
Cancer
Homologous Recombination
Non-homologous End Joining (NHEJ)
Double-strand Breaks (DSBs)
Mitotic Cells
DNA Replication
Oxidative Lesions
Ionizing Radiation (IR)
Exogenous DNA Damaging Agents
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