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准备使用一个不连续Percoll蔗糖密度梯度的从小鼠皮质Synaptoneurosomes
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
Please note that all translations are automatically generated.
Click here for the English version.
准备使用一个不连续Percoll蔗糖密度梯度的从小鼠皮质Synaptoneurosomes
DOI:
10.3791/3196-v
•
08:30 min
•
September 17, 2011
•
Pamela R. Westmark
,
Cara J. Westmark
,
Athavi Jeevananthan
,
James S. Malter
1
Department of Pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities
,
University of Wisconsin
,
2
Department of Biochemistry, Waisman Center for Developmental Disabilities
,
University of Wisconsin
Chapters
00:05
Title
01:23
Gradient Preparation
02:20
Mouse Dissection
03:27
Preparation of Homogenate and Synaptoneurosomes
05:51
Results: Synaptoneurosome Using a Discontinuous Percoll-sucrose Density Gradient
08:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个准备从小鼠大脑皮质翻译活跃,完好synaptoneurosomes(SNS)的方法来描述。该方法采用一个不连续Percoll蔗糖密度梯度,允许快速制备活跃的SNS。
Tags
Synaptoneurosomes
Mouse Cortex
Discontinuous Percoll-Sucrose Density Gradient
Homogenization
Fractionation
Resealed Vesicles
Isolated Terminals
Synaptic Transmission
Pre- And Postsynaptic Characteristics
Neuronal Signaling
Neurotransmitters
Ficoll
Cytotoxic
Centrifugation
Filtration
Mechanical Damage
Percoll-sucrose Gradient Method
Translationally Active SNs
Isotonic Conditions
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