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鼠标应用结扎Peyer氏修补肠袢含量评价M细胞的细菌吸收
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Immunology and Infection
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JoVE Journal Immunology and Infection
Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
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鼠标应用结扎Peyer氏修补肠袢含量评价M细胞的细菌吸收

DOI:
10.3791/3225-v

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05:59 min

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December 17, 2011

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, ,
1Laboratory for Epithelial Immunobiology,RIKEN Research Center for Allergy and Immunology

Chapters

  • 00:05Title
  • 01:12Preparation of Bacterial Cells
  • 02:26Ligated Peyer’s Patch Loop Assay
  • 03:42Whole Mount Staining and Confocal Microscopic Analysis of Peyer’s Patch
  • 05:12Localization of Fluorescent S. Typhimurium and GP2 in M Cells
  • 05:36Conclusion

Summary

Automatic Translation

Automatic Translation

在一个专门的卵泡相关Peyer氏斑上皮M细胞在肠道相关淋巴组织的粘膜免疫监视中发挥的重要作用。在这里，我们描述了细菌transcytosis M细胞的评价方法<em>在体内</em>。这种方法提供了一种方法，以了解在免疫系统中的M -细胞功能。

Tags

Mouse Ligated Peyer’s Patch Intestinal Loop AssayBacterial UptakeM CellsMucosal Immune ResponseMucosal AntigensAntigen TranscytosisSpecialized Epithelial M CellsMacromolecular TranscytosisAntigen-specific Mucosal Immune ResponsesGlycoprotein 2 (Gp2)EnterocytesCommensal BacteriaPathogenic Bacteria

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