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Bioengineering
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刀口扫描显微镜的样品制备,图像和分析协议
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
刀口扫描显微镜的样品制备,图像和分析协议
DOI:
10.3791/3248-v
•
10:25 min
•
December 09, 2011
•
Yoonsuck Choe
,
David Mayerich
,
Jaerock Kwon
,
Daniel E. Miller
,
Chul Sung
,
Ji Ryang Chung
,
Todd Huffman
,
John Keyser
,
Louise C. Abbott
1
Department of Computer Science and Engineering
,
Texas A&M University
,
2
Beckman Institute for Advanced Science and Technology
,
University of Illinois
,
3
Department of Electrical and Computer Engineering
,
Kettering University
,
4
3Scan
,
5
Department of Veterinary Integrative Biosciences
,
Texas A&M University
Chapters
00:05
Title
01:27
Specimen Preparation: Golgi-Cox Method
03:20
Specimen Preparation: Nissl Method
04:39
Specimen Preparation: India Ink Method
05:37
KESM Setup and Imaging
06:38
Image Processing, Data Visualization and Analysis
08:12
Representative Results of KESM
10:00
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
从大脑标本制备连续切片成像,用刀口扫描显微镜,数据可视化和分析的全过程描述。这项技术是目前用于收购鼠脑数据,但它是适用于其他器官,其他物种。
Tags
Specimen Preparation
Imaging
Analysis Protocols
Knife-edge Scanning Microscopy
High-throughput Microscopy
High-resolution Microscopy
3D Microscopy
Neuroanatomical Data
Submicrometer Resolution
Whole-brain-scale Data
Neuronal Networks
Vascular Networks
Cell Body Distribution
Connectomics Research
Microcirculation Research
Hemodynamic Research
Stereology Research
Fixing
Staining
Embedding
KESM Configuration And Setup
Sectioning And Imaging With KESM
Image Processing
Data Preparation
Data Visualization
Data Analysis
Article
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