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Etablering af Epstein-Barr virus Vækst-transformerede lymfoblastoide cellelinjer
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Etablering af Epstein-Barr virus Vækst-transformerede lymfoblastoide cellelinjer

DOI:
10.3791/3321-v

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06:38 min

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November 08, 2011

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, , , ,
1Stony Brook Children’s Hospital,State University of New York at Stony Brook, 2Department of Pediatrics,State University of New York at Stony Brook, 3Department of Molecular Genetics,State University of New York at Stony Brook, 4Department of Microbiology,State University of New York at Stony Brook

Chapters

  • 01:35Isolation of Peripheral Blood Mononuclear Cells (PBMC)
  • 02:53Infection with Epstein-Barr Virus (EBV)
  • 03:47Expansion and Cryopreservation of Lymphoblastoid Cell Lines (LCL)
  • 04:46Success of Outcome can be Predicted by Flow Cytometric Analysis of Cells
  • 05:58Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en metode til at generere transformeret B-cellelinjer med Epstein-Barr virus. Vi har også illustrere en roman assay, der kan identificere B-celler bestemt til at undergå transformation så tidligt som tre dage efter smitte.

Tags

Epstein-Barr VirusGrowth-transformed Lymphoblastoid Cell LinesEBV InfectionB Cell ProliferationImmortalizationEBV LatencyVirus-driven B Cell ProliferationTumorigenesisImmunologic AssaysHuman Monoclonal AntibodiesMitogensPhytohemagglutininLipopolysaccharidePokeweed MitogenCyclosporin AT Cell-mediated KillingHigh Titer EBVImmunosuppressive AgentPeripheral Blood Mononuclear Cells

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