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Etablering af Epstein-Barr virus Vækst-transformerede lymfoblastoide cellelinjer
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
DOI:

06:38 min

November 08, 2011

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Chapters

  • 01:35Isolation of Peripheral Blood Mononuclear Cells (PBMC)
  • 02:53Infection with Epstein-Barr Virus (EBV)
  • 03:47Expansion and Cryopreservation of Lymphoblastoid Cell Lines (LCL)
  • 04:46Success of Outcome can be Predicted by Flow Cytometric Analysis of Cells
  • 05:58Conclusion

Summary

Automatic Translation

Vi beskriver en metode til at generere transformeret B-cellelinjer med Epstein-Barr virus. Vi har også illustrere en roman assay, der kan identificere B-celler bestemt til at undergå transformation så tidligt som tre dage efter smitte.

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