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JoVE Journal
Biology

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单次读取和配对的最终从10微克总RNA的mRNA - Seq的Illumina的图书馆
 
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单次读取和配对的最终从10微克总RNA的mRNA - Seq的Illumina的图书馆

Article DOI: 10.3791/3340
October 27th, 2011

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Summary October 27th, 2011

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在这里,我们描述了读取和配对的单端Illumina公司的mRNA - Seq的测序基础上的T7线性RNA扩增的基因表达分析库的制备方法。该协议只需要10微克启动总RNA,并产生了高度一致库代表整个笔录。

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