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醋酸形成醋酸酶激酶活性的直接检测
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Biology
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JoVE Journal
Biology
Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
Please note that all translations are automatically generated.
Click here for the English version.
醋酸形成醋酸酶激酶活性的直接检测
DOI:
10.3791/3474-v
•
05:51 min
•
December 19, 2011
•
Matthew L. Fowler
,
Cheryl J. Ingram-Smith
,
Kerry S. Smith
1
Department of Genetics and Biochemistry
,
Clemson University
Chapters
00:05
Title
01:10
Acetyl Phosphate Standard Curve
02:19
Assay for Acetate Kinase Activity
03:31
The Direct Assay for Acetate Kinase Activity
05:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
醋酸激酶活性测定方法的描述。本实验利用一个直接反应在醋酸形成的方向确定不同磷受体酶的活性和乙酸激酶动力学。此外,这种方法可以用于化验等乙酰磷酸盐或乙酰CoA利用酶。
Tags
Acetate Kinase
Acetate-forming Activity
Enzyme
Enzyme Superfamily
Phosphorylation
ATP
Acetyl Phosphate
Bacteria
Archaea
Eukarya
Methanosarcina Thermophila
Entamoeba Histolytica
Amoebic Dysentery
Hydroxamate Assay
NADH
NAD+
Pyruvate Kinase
Lactate Dehydrogenase
Inorganic Phosphate
Hydroxylamine
ATP Formation
ADP
NADP+
NADPH
Hexokinase
Glucose 6-phosphate Dehydrogenase
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