A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:10Acetyl Phosphate Standard Curve
- 02:19Assay for Acetate Kinase Activity
- 03:31The Direct Assay for Acetate Kinase Activity
- 05:23Conclusion
En metode til bestemmelse af acetat kinase aktivitet er beskrevet. Denne analyse anvender en direkte reaktion til bestemmelse af enzymaktivitet og kinetik af acetat kinase i acetat-dannende retning med forskellige phosphoryl receptorer. Desuden kan metoden anvendes til analysering andre acetyl fosfat eller acetyl-CoA udnytte enzymer.
Tags
Acetate KinaseAcetate-forming ActivityEnzymeEnzyme SuperfamilyPhosphorylationATPAcetyl PhosphateBacteriaArchaeaEukaryaMethanosarcina ThermophilaEntamoeba HistolyticaAmoebic DysenteryHydroxamate AssayNADHNAD+Pyruvate KinaseLactate DehydrogenaseInorganic PhosphateHydroxylamineATP FormationADPNADP+NADPHHexokinaseGlucose 6-phosphate Dehydrogenase
