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Immunology and Infection
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重组细小病毒与腺病毒派生系统的帮助下高效生产
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Immunology and Infection
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Immunology and Infection
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
Please note that all translations are automatically generated.
Click here for the English version.
重组细小病毒与腺病毒派生系统的帮助下高效生产
DOI:
10.3791/3518-v
•
13:47 min
•
April 23, 2012
•
Nazim El-Andaloussi*
1,2
,
Barbara Leuchs*
1
,
Serena Bonifati
2
,
Jean Rommelaere
2
,
Antonio Marchini
2
1
Tumour Virology Division F010
,
German Cancer Research Center (DKFZ)
,
2
Inserm Unit 701
,
German Cancer Research Center (DKFZ)
Chapters
00:05
Title
03:28
Production of recPVs via Transfection
07:09
Production of recPVs via Infection
11:00
Results: Improved recPV Production by Adenovirus Genomic Elements
13:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述只对细胞的感染,从而提高重组细小的生产效率超过100倍相比,在使用中的其他协议为基础的协议。此协议依赖于使用一种新型腺病毒含有的细小副总裁转录单位(AD-VP)5,基于辅助。
Tags
Recombinant Parvovirus
Adenovirus-derived Systems
Rodent Parvoviruses
PV Genome
Promoters P4 And P38
Non-structural Proteins (NS1 And NS2)
Capsid Proteins (VP1 And VP2)
Anticancer Agents
Oncolytic Abilities
Oncosuppressive Abilities
NS1 Cytotoxicity
Therapeutic Transgene
RecPVs Vector
Viral DNA Amplification
Packaging
Producer Cells (HEK293T)
PCMV-VP Vector
Replication Defective
Oncotoxic Activities
Anti-cancer Agents
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