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Bioengineering
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警队复用单分子蛋白水解测量磁性镊子
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
Please note that all translations are automatically generated.
Click here for the English version.
警队复用单分子蛋白水解测量磁性镊子
DOI:
10.3791/3520-v
•
10:08 min
•
July 25, 2012
•
Arjun S. Adhikari
,
Jack Chai
,
Alexander R. Dunn
1
Department of Chemical Engineering
,
Stanford University
Chapters
00:05
Title
01:40
Flow Cell Preparation
02:52
Magnetic Tweezers Set-up and Calibration
05:55
Collagen Peptide Attachment to Flow Cells
07:00
Force Proteolysis Assay
07:56
Results: The Effect of Force on Enzymatic Proteolysis
08:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这篇文章中,我们描述了利用磁性镊子,在一个高度并行的方式在单分子水平研究的酶水解的影响力。
Tags
Multiplexed
Single-molecule
Force
Proteolysis
Measurements
Magnetic Tweezers
Generation
Detection
Mechanical Forces
Cell Physiology
Cancer Metastasis
Atherogenesis
Wound Healing
Extracellular Matrix (ECM)
Enzymatically Remodel
Forces On ECM
Biological Importance
Medical Importance
Single Molecule Techniques
Optical Trapping
Atomic Force Microscopy
Magnetic Tweezers (MT)
Low Cost
High Throughput
PN (piconewton)
Millisecond Temporal Resolution
Enzyme Mechanism
Highly Parallelizable Assay
Trimeric Collagen Peptide
Matrix Metalloproteinase 1 (MMP-1)
Substrates And Proteases
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