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Bioengineering
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磁気ピンセットを用いた多重化単分子フォースタンパク質分解測定
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
Please note that all translations are automatically generated.
Click here for the English version.
磁気ピンセットを用いた多重化単分子フォースタンパク質分解測定
DOI:
10.3791/3520-v
•
10:08 min
•
July 25, 2012
•
Arjun S. Adhikari
,
Jack Chai
,
Alexander R. Dunn
1
Department of Chemical Engineering
,
Stanford University
Chapters
00:05
Title
01:40
Flow Cell Preparation
02:52
Magnetic Tweezers Set-up and Calibration
05:55
Collagen Peptide Attachment to Flow Cells
07:00
Force Proteolysis Assay
07:56
Results: The Effect of Force on Enzymatic Proteolysis
08:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
この記事では、高度に並列化可能な方法で単一分子レベルでのタンパク質分解酵素に作用する力の影響を研究するために磁気ピンセットの使用方法について説明します。
Tags
Multiplexed
Single-molecule
Force
Proteolysis
Measurements
Magnetic Tweezers
Generation
Detection
Mechanical Forces
Cell Physiology
Cancer Metastasis
Atherogenesis
Wound Healing
Extracellular Matrix (ECM)
Enzymatically Remodel
Forces On ECM
Biological Importance
Medical Importance
Single Molecule Techniques
Optical Trapping
Atomic Force Microscopy
Magnetic Tweezers (MT)
Low Cost
High Throughput
PN (piconewton)
Millisecond Temporal Resolution
Enzyme Mechanism
Highly Parallelizable Assay
Trimeric Collagen Peptide
Matrix Metalloproteinase 1 (MMP-1)
Substrates And Proteases
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