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随机使用时移视频显微镜细胞迁移的定量分析
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Biology
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JoVE Journal
Biology
Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
随机使用时移视频显微镜细胞迁移的定量分析
DOI:
10.3791/3585-v
•
07:27 min
•
May 13, 2012
•
Prachi Jain
,
Rebecca A. Worthylake
,
Suresh K. Alahari
3
1
Department of Biochemistry and Molecular Biology
,
LSU School of Medicine
,
2
Department of Oral Biology
,
LSU School of Dentistry
,
3
Stanley S. Scott Cancer Center
,
LSU School of Medicine
Chapters
00:05
Title
01:26
Preparation of Plate for Image Acquisition
02:17
Microscope Setup and Automated Image Acquisition
04:44
Image Processing and Analysis of Migration: Calculation of Speed and Displacement
05:39
Results: Random Migration Analysis and Videography
06:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这种方法允许在不同的细胞迁移参数,如速度,位移和速度的实时性和定量测量细胞的监测。实时方式与传统方法不同的是,这不是基于端点的定量迁移测量;相反,它允许不同的参数,不断监测和计算。
Tags
Quantitative Analysis
Random Migration
Cells
Time-lapse Video Microscopy
Cell Migration
Embryonic Development
Immune System Function
Tumor Invasion
Chemotactic Signal
Intrinsic Cues
Motility Machinery
Local Environment
Polarization
Protrusions
Boyden Chamber Migration Assay
Chemotaxis
End Point Result
Individual Migration Parameters
Morphological Changes
Cancer Cell Migration
Cancer Cell Invasion
Platelets
Platelet Function
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