Journal
/
/
随机使用时移视频显微镜细胞迁移的定量分析
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy
DOI:

07:27 min

May 13, 2012

, ,

Chapters

  • 00:05Title
  • 01:26Preparation of Plate for Image Acquisition
  • 02:17Microscope Setup and Automated Image Acquisition
  • 04:44Image Processing and Analysis of Migration: Calculation of Speed and Displacement
  • 05:39Results: Random Migration Analysis and Videography
  • 06:50Conclusion

Summary

Automatic Translation

这种方法允许在不同的细胞迁移参数,如速度,位移和速度的实时性和定量测量细胞的监测。实时方式与传统方法不同的是,这不是基于端点的定量迁移测量;相反,它允许不同的参数,不断监测和计算。

Related Videos

Read Article