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跨物种移植神经嵴迁移和分化的分析

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

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跨物种移植神经嵴迁移和分化的分析

DOI:

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09:03 min

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February 07, 2012

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Shannon L. Griswold, Peter Y. Lwigale

¹Department of Biochemistry and Cell Biology,Rice University

Chapters

00:05Title
01:26Incubate Chick and Quail Eggs to the Desired Stage
01:57Prepare Eggs for Windowing and Dissection
06:15Grafting the Tissue
07:21Immunofluorescent Detection of Quail-specific Nuclear Antigen QCPN in Donor-derived Cells in the Chimeric Embryo
08:35Conclusion

Summary

Automatic Translation

描述的分析禽流鹌鹑鸡嵌合胚胎的神经嵴细胞的迁移和最终命运的方法。此方法是一种简单明了的技术追踪神经嵴细胞迁移和分化的过程中，否则难以区分，在未添加鸡胚。

Tags

Neural Crest Migration Differentiation Cross-species Transplantation Avian Embryos Quail-chick Chimeras Migratory Neural Crest Cells Epithelial To Mesenchymal Transition Cell Types Cartilage Melanocytes Neurons Glia Multipotent Cells Rostro-caudal Axis Neighboring Cells Microenvironment Patterning Organogenesis

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