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Analyse af Neural Crest migrering og differentiering af Cross-arter Transplantation
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Chapters
- 00:05Title
- 01:26Incubate Chick and Quail Eggs to the Desired Stage
- 01:57Prepare Eggs for Windowing and Dissection
- 06:15Grafting the Tissue
- 07:21Immunofluorescent Detection of Quail-specific Nuclear Antigen QCPN in Donor-derived Cells in the Chimeric Embryo
- 08:35Conclusion
En metode til analyse af indvandring og eventuel skæbne aviære neurale crest celler i vagtel-chick kimære embryoner er beskrevet. Denne fremgangsmåde er en enkel og ligetil teknik til sporing crista neuralis-celler under migrering og differentiering, som ellers er vanskelige at skelne i en manipuleret chick embryo.
