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Analyse av Neural Crest Migrasjon og differensiering av Cross-arter Transplantation
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation
DOI:

09:03 min

February 07, 2012

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Chapters

  • 00:05Title
  • 01:26Incubate Chick and Quail Eggs to the Desired Stage
  • 01:57Prepare Eggs for Windowing and Dissection
  • 06:15Grafting the Tissue
  • 07:21Immunofluorescent Detection of Quail-specific Nuclear Antigen QCPN in Donor-derived Cells in the Chimeric Embryo
  • 08:35Conclusion

Summary

Automatic Translation

En tilnærming for å analysere migrasjon og eventuell skjebne av aviær neural crest cellene i vaktel-chick chimeric embryoer er beskrevet. Denne metoden er en enkel og grei teknikk for sporing neural crest cellene under trekket og differensiering som ellers er vanskelig å skille innenfor en unmanipulated kylling embryo.

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