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1Laboratory of Molecular Immunology and Immunotherapy,Blood Research Institute, 2Department of Microbiology,Mount Sinai School of Medicine , 3Laboratory of Molecular Genetics,Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children’s Hospital of Wisconsin,Medical College of Wisconsin , 6Division of Hematology and Oncology, Dept Medicine,Medical College of Wisconsin
Chapters
- 00:05Title
- 02:26Intra-nasal Infection of Mice with Influenza and Collection of Bronchio-Alveolar Lavage Fluid
- 03:22Infection of MDCK Cells with Virus and Detection of Viral M2 Protein with the LI-COR Odyssey
- 08:09Quantification of Influenza Viral Titer using Infra-red Staining and Detection
- 09:16Conclusion
Diese Methode beschreibt die Verwendung von Infrarot-Farbstoff basierende Imaging-System für den Nachweis von H1N1 in bronchioalveolar Lavage (BAL) Flüssigkeit von infizierten Mäuse mit einer hohen Empfindlichkeit. Diese Methodik in einem 96 durchgeführt werden kann - oder 384-Well-Platte, erfordert <10 ul Volumen von Testmaterial und hat das Potenzial für die gleichzeitige Screening von mehreren Erregern.
Tags
High-throughput Detection MethodInfluenza VirusPrecise DiagnosisInfecting StrainRapid ScreeningClinical SamplesPandemic InfectionsSerologic TestingPolymerase Chain ReactionDirect Specimen ImmunofluorescenceCell CultureNovel Diagnostic TechniqueLive Influenza VirusesMouse-adapted Human A/PR/8/34 (PR8H1N1) VirusMDCK CellsViral ProteinsAnti-M2 AntibodyHemagglutinin 1Diagnostic AssaysIR-dye-conjugated Secondary AntibodiesAntigen-specific Fluorescence IntensityLI-COR Odyssey-based IR Scanner
