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Konstruere en Kollagen Hydrogel for levering av Stem Cell-lastet Chitosan Mikrokuler
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Chapters
- 00:05Title
- 01:25Isolating Adipose-derived Stem Cells
- 02:40Preparing Chitosan Microspheres
- 04:00Quantify Free Amino Groups in CSM
- 05:20Loading ASC in CSM
- 06:11Quantify ASC Loading and Cell Viability in CSM
- 07:17Characterizing ASC-CSM-embedded Collagen Gel
- 08:04ASC-CSM-collagen Gel
- 08:55Conclusion
Et stort hinder i dagens stilk cellen terapi er å bestemme den mest effektive metoden for å levere disse cellene til verten vev. Her beskriver vi en kitosan-basert levering metode som er effektiv og enkel i tilnærmingen, samtidig som adipose-avledet stilk celler for å opprettholde sin multipotency.
Tags
Collagen HydrogelStem Cell-loaded Chitosan MicrospheresRegenerative MedicineImplantation SiteMechanical PropertiesVascularizationExtracellular MatrixBiocompatible MaterialsChemical CompositionMechanical StrengthPermeabilityOxygen And NutrientsChitosanCationic PolysaccharideChemical ModificationIn Vivo MacromoleculesGlycosaminoglycan PortionSubstrate For Cell AdhesionMigration And ProliferationAdipose-derived Stem Cells (ASC)Three-dimensional Scaffold
