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检测DNA损伤体外半人工分子机器,其使用的大会
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
DOI:

08:56 min

January 11, 2012

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Chapters

  • 00:05Title
  • 02:23Preparation of Tissue Sections
  • 04:02Molecular Machine Assembly
  • 04:59Dual Labeling 5’OH- and 5’PO4- DNA Breaks in Tissue Sections
  • 06:41Results of Detecting DNA Breaks with a Molecular Machine
  • 07:57Conclusion

Summary

Automatic Translation

我们证明由拓扑异构酶蛋白驱动的分子尺度器件的组装和应用。构造是一个标签由组织切片中的DNA断裂的两个主要类型,其两端连接两个不同的荧光团的生物分子传感器。

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