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In vitro Montering af Semi-kunstige Molekylær Machine og dens anvendelse til påvisning af DNA-skader
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Bioengineering
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JoVE Journal Bioengineering
In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

In vitro Montering af Semi-kunstige Molekylær Machine og dens anvendelse til påvisning af DNA-skader

DOI:
10.3791/3628-v

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08:56 min

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January 11, 2012

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,
1Neurosurgery,Baylor College of Medicine , 2Michael E. DeBakey Veterans Affairs Medical Center, 3Molecular & Cellular Biology,Baylor College of Medicine

Chapters

  • 00:05Title
  • 02:23Preparation of Tissue Sections
  • 04:02Molecular Machine Assembly
  • 04:59Dual Labeling 5’OH- and 5’PO4- DNA Breaks in Tissue Sections
  • 06:41Results of Detecting DNA Breaks with a Molecular Machine
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

Vi viser monteringen og anvendelsen af ​​en molekylær-skala-enhed drevet af en topoisomerase protein. Det begreb er en bio-molekylære sensor, som etiketter to store typer af DNA-brud i vævssnit ved at knytte to forskellige fluorophores til deres mål.

Tags

In Vitro AssemblySemi-artificial Molecular MachineDNA Damage DetectionBio-molecular MachinesArtificial Molecular MachinesMolecular MotorsNanoworld EnvironmentsNanotechnology ProductsBio-enabled ConstructDesigner Fluorescent Enzyme-substrate ComplexMolecular MachineFluorescent Dual HairpinLabeling DNA Da

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