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神经母细胞含量：从区分神经干细胞后代使用流式细胞仪，神经祖细胞的生成和富集含量
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Neuroscience
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JoVE Journal Neuroscience
The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry
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神经母细胞含量：从区分神经干细胞后代使用流式细胞仪，神经祖细胞的生成和富集含量

DOI:
10.3791/3712-v

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15:40 min

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April 22, 2012

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, , ,
1Department of Neurosurgery,The University of Florida, 2Laboratory for Stem Cell Research, Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran

Chapters

  • 00:02Title
  • 01:46Starting Neuroblast Assay Culture
  • 04:07Switching Neuroblast Assay Medium
  • 04:55Cell Preparation for Flow Cytometry
  • 06:42Staining for PSA-NCAM
  • 08:31Isolation of Neuronal Progenitor Cells Using Flow Cytometry
  • 11:34Plating Sorted Cells
  • 13:12Representative Results
  • 14:34Conclusion

Summary

Automatic Translation

Automatic Translation

这个视频协议，表明了新方法的产生和后续的纯化，根据自己的身体（大小和内部的粒度）和荧光性能，使用流式细胞仪技术从可再生来源的神经干细胞（NS​​Cs）的神经祖细胞。

Tags

Neuroblast AssayGeneration Of Neuronal Progenitor CellsDifferentiating Neural Stem CellsFlow CytometryNeurosphere AssayCentral Nervous SystemAstrocytesOligodendrocytesNeuronsIn Vitro StudiesDrug ScreeningNeurotoxicologyElectrophysiologyCell Replacement TherapyHeterogeneity Of NSC ProgenyLow Production Of Neurons And OligodendrocytesPredominance Of AstrocytesClinical ApplicationsPurification Of Immature NeuronsFluorescence Activated Cell Sorting (FACS)Murine NSC ProgenyE14 Mouse Ganglionic Eminences

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