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Analytiske teknikker til analyse af nitrogenoxid Bioaktivitet
JoVE Journal
Medicine
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JoVE Journal Medicine
Analytical Techniques for Assaying Nitric Oxide Bioactivity
DOI:

11:28 min

June 18, 2012

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Chapters

  • 00:05Title
  • 01:59Whole Blood Preparations
  • 03:05Preparing the Plasma for Biochemical Analysis
  • 04:26Preparing the Red Blood Cells for Biochemical Analysis
  • 05:18Tissue Extraction and Preparation
  • 06:02Aortic Ring Isolations
  • 08:10Analyzing Endothelial Function
  • 09:25Dose Response to Acetylcholine
  • 10:11Conclusion

Summary

Automatic Translation

Den endogene produktion af nitrogenoxid (NO) regulerer en lang række biologiske funktioner. Det bliver mere og mere klart, at afbrydelse eller dysregulering af NO baserede signalering er involveret i mange sygdomme hos mennesker. Metoder til at kvantificere relevante INGEN metabolitter kan give nye diagnostiske eller prognostiske biomarkører for human sygdom.

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