A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:59Whole Blood Preparations
- 03:05Preparing the Plasma for Biochemical Analysis
- 04:26Preparing the Red Blood Cells for Biochemical Analysis
- 05:18Tissue Extraction and Preparation
- 06:02Aortic Ring Isolations
- 08:10Analyzing Endothelial Function
- 09:25Dose Response to Acetylcholine
- 10:11Conclusion
Den endogene produktion af nitrogenoxid (NO) regulerer en lang række biologiske funktioner. Det bliver mere og mere klart, at afbrydelse eller dysregulering af NO baserede signalering er involveret i mange sygdomme hos mennesker. Metoder til at kvantificere relevante INGEN metabolitter kan give nye diagnostiske eller prognostiske biomarkører for human sygdom.
Tags
Analytical TechniquesAssayingNitric Oxide BioactivityDetectionQuantificationSignaling MoleculesBlood Pressure RegulationImmune Response RegulationNeural Communication RegulationAccurate DetectionQuantification In Biological MatricesPhysiological Half LifeAlternative StrategiesDetection Of Reaction ProductsNO MetabolitesBiological CompartmentsIn Vivo NO ProductionBioavailabilityMetabolismWhole Body NO StatusDiagnostic UtilityPrognostic UtilityNO Biomarkers
