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小鼠气管基底细胞和粘膜下腺体的导管细胞分离
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Biology
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JoVE Journal
Biology
Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea
Please note that all translations are automatically generated.
Click here for the English version.
小鼠气管基底细胞和粘膜下腺体的导管细胞分离
DOI:
10.3791/3731-v
•
11:52 min
•
September 14, 2012
•
Ahmed E. Hegab
,
Vi Luan Ha
,
Yasser S. Attiga
,
Derek W. Nickerson
,
Brigitte N. Gomperts
1
Department of Pediatrics
,
David Geffen School of Medicine at UCLA
Chapters
00:05
Title
01:45
Dissection of Trachea
02:46
Cleaning and Cutting the Trachea
03:45
Enzyme Digestion and Processing into Single Cell Suspension
06:42
Staining for FACS and Sorting
07:23
Processing Sorted Cells for in vitro Culture and in vivo Model
09:40
Representative Data
11:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们展示了我们的协议,用于隔离的基础和粘膜下腺体导管细胞从小鼠气管。我们还表明,该方法的干细胞注射到背鼠标脂肪垫,以创建一个<em在体内</em>模式,粘膜下腺体的再生。
Tags
Isolation
Basal Cells
Submucosal Gland Duct Cells
Mouse Trachea
Large Airways
Repair Mechanism
Stem Cells
In Vitro Model Systems
In Vivo Model Systems
Mechanisms Of Repair And Regeneration
Airway Diseases
Cystic Fibrosis
Asthma
Chronic Obstructive Pulmonary Disease
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