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小鼠气管基底细胞和粘膜下腺体的导管细胞分离
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea
DOI:

11:52 min

September 14, 2012

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Chapters

  • 00:05Title
  • 01:45Dissection of Trachea
  • 02:46Cleaning and Cutting the Trachea
  • 03:45Enzyme Digestion and Processing into Single Cell Suspension
  • 06:42Staining for FACS and Sorting
  • 07:23Processing Sorted Cells for in vitro Culture and in vivo Model
  • 09:40Representative Data
  • 11:19Conclusion

Summary

Automatic Translation

在这里,我们展示了我们的协议,用于隔离的基础和粘膜下腺体导管细胞从小鼠气管。我们还表明,该方法的干细胞注射到背鼠标脂肪垫,以创建一个<em在体内</em>模式,粘膜下腺体的再生。

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