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抛光和钢筋,颅骨变薄窗口,为长期的小鼠脑成像
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
Please note that all translations are automatically generated.
Click here for the English version.
抛光和钢筋,颅骨变薄窗口,为长期的小鼠脑成像
DOI:
10.3791/3742-v
•
09:49 min
•
March 07, 2012
•
Andy Y. Shih
,
Celine Mateo
,
Patrick J. Drew
3
,
Philbert S. Tsai
,
David Kleinfeld
4
1
Department of Physics
,
University of California, San Diego
,
2
Department of Engineering Science and Mechanics
,
Pennsylvania State University
,
3
Department of Neurosurgery
,
Pennsylvania State University
,
4
Section of Neurobiology
,
University of California, San Diego
Chapters
00:05
Title
01:40
Surgery Preparation and Animal Mounting
04:02
The Generation of a Polished and Reinforced Thinned-skull Window
07:14
Imaging Preparation
08:20
Representative Results for Long-term Imaging and optical Manipulation of the Mouse Cortex
09:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个方法,形成一个横跨毫米,是没有大脑的炎症个月稳定在小鼠颅骨的影像窗口。这种方法非常适合于使用双光子显微镜的血液流通,细胞动力学,细胞/血管结构的纵向研究。
Tags
Polished And Reinforced Thinned-skull Window
Long-term Imaging
Mouse Brain
In Vivo Imaging
Cortical Function
Optical Access
Intracranial Environment
PoRTS Window
Optical Clarity
Bone Regrowth Inhibition
Light Scattering Reduction
Inflammation Reduction
Two-photon Laser Scanning Microscopy
Cerebral Blood Flow
Cellular Function
Anesthetized Preparations
Awake Preparations
Optogenetics
Blood Flow Disruption
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