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Journal
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Immunology and Infection
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在实验性自身免疫性脑脊髓炎(EAE)抗性品系小鼠继转移和抗原挑战克服反应迟钝
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
Please note that all translations are automatically generated.
Click here for the English version.
在实验性自身免疫性脑脊髓炎(EAE)抗性品系小鼠继转移和抗原挑战克服反应迟钝
DOI:
10.3791/3778-v
•
08:38 min
•
April 09, 2012
•
Michael K. Shaw
,
Xiao-qing Zhao
,
Harley Y. Tse
1
Department of Medicine, Section of Cardiology
,
St. John-Providence Health System
,
2
Department of Immunology and Microbiology
,
Wayne State University School of Medicine
Chapters
00:05
Title
01:28
Preparing CFA-emulsified Antigen
03:08
Immunization of Donor Mice
03:51
Isolation of Lymph Node Cells after Immunization and Re-stimulation with Antigen
06:07
Harvest of Re-stimulated Lymph Node Cells and Adoptive Transfer
06:47
Antigenic Challenge of Recipient Mice and Development of Disease
07:31
Results: Kinetics of Disease Progression Following Immunization, Adoptive Transfer and Challenge
07:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
某些鼠标菌株能够抵抗与髓鞘碱性蛋白诱导实验性自身免疫性脑脊髓炎(EAE)。这里描述的是一个简单的免疫协议,扭转反应迟钝和麻痹性疾病引起几个典型的EAE的耐鼠标污渍。
Tags
Experimental Autoimmune Encephalomyelitis
EAE
Mouse Strains
Adoptive Transfer
Antigenic Challenge
Central Nervous System
Mononuclear Cells
Paralytic Disease
Multiple Sclerosis
Genetic Control
C57BL/6 Mice
Myelin Basic Protein
Antigen Processing
Antigen Presentation
Encephalitogenic T Cell Clones
Cellular Adoptive Transfer System
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