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荧光显微镜筛选和新一代测序:参与细胞器完整性的基因鉴定的有用工具
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Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
Please note that all translations are automatically generated.
Click here for the English version.
荧光显微镜筛选和新一代测序:参与细胞器完整性的基因鉴定的有用工具
DOI:
10.3791/3809-v
•
12:42 min
•
April 13, 2012
•
Giovanni Stefano
,
Luciana Renna
,
Federica Brandizzi
1
DOE Plant Research Laboratory
,
Michigan State University
Chapters
00:05
Title
01:20
Ethyl Methane Sulfonate (EMS) Treatment
04:25
Confocal Screening of M2 and M3 Populations
05:35
Mapping
09:10
Results: Mutation Mapping using Genechip
Arabidopsis
ATH1 Genome Array Hybridization and R Software Analysis
12:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在细胞生物学的一个基本任务是定义机制的基础,使真核细胞的细胞器的身份。在这里,我们提出了一个方法,以确定该基因的植物细胞器,利用荧光显微镜和下一代测序工具的形态和功能的完整性负责。
Tags
Fluorescence-microscopy Screening
Next-generation Sequencing
Identification Of Genes
Organelle Integrity
Arabidopsis Seedlings
Recessive Mutations
Subcellular Distribution
Tagged Fluorescent Marker
Secretory Pathway
Genetic Studies
Genome Size
Generation Time
Conservation Of Molecular Mechanisms
Array Genotyping
Alternative Mapping Method
Molecular Markers
Proteins Influencing Organelle Integrity
Endoplasmic Reticulum (ER)
Plant Cell Organelles
Molecular Basis
Article
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