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Journal / Neuroscience / Multiphoton Mikroskopi av Rensat mushjärna…
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JoVE Journal Neuroscience
Multiphoton Mikroskopi av Rensat mushjärna uttrycker YFP
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Multiphoton Mikroskopi av Rensat mushjärna uttrycker YFP

Article DOI: 10.3791/3848-v • 10:04 min • September 23rd, 2012
September 23rd, 2012 •
*1, *1, 1,2, 1
1Department of Biomedical Engineering, Yale University, 2Department of Biomedical Engineering, Louisiana Tech University
* These authors contributed equally

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Multiphoton mikroskopi av hela mus organ är möjlig genom optiskt rensa organet före avbildning, men inte alla protokoll bevara fluorescenssignalen av fluorescerande proteiner. Använda en optisk clearing metod med etanol-baserad uttorkning och bensylalkohol: bensylbensoat clearing visar vi högupplösta multiphoton bilder av hela mushjärna uttrycker YFP.

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Neurovetenskap Medicinsk teknik molekylärbiologi multiphoton mikroskopi mus hjärna röjning YFP fluroescence
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