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Fremstilling af myeloide afledte suppressorceller (MDSC) fra naive og pancreas Tumorbærende mus under anvendelse af flowcytometri, og Automatiseret magnetisk aktiveret cellesortering (AutoMACS)
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JoVE Journal Medicine
Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)

Fremstilling af myeloide afledte suppressorceller (MDSC) fra naive og pancreas Tumorbærende mus under anvendelse af flowcytometri, og Automatiseret magnetisk aktiveret cellesortering (AutoMACS)

DOI:
10.3791/3875-v

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14:15 min

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June 18, 2012

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, , ,
1Department of Molecular Medicine,University of South Florida Morsani College of Medicine

Chapters

  • 00:05Title
  • 03:11Generation of a Single-cell Suspension of Leukocytes from Spleens
  • 05:09Cell Surface Staining/Immunophenotyping of MDSC using Flow Cytometry
  • 09:01Magnetic Enrichment of Gr-1+ Leukocytes
  • 12:06Post-sort Analysis of Gr-1-enriched Leukocytes
  • 12:51AutoMACS Enrichment of Naive Gr-1+ Leukocytes for MDSC FACS Sorting
  • 13:40Conclusion

Summary

Automatic Translation

Automatic Translation

Dette er en hurtig og omfattende fremgangsmåden immunfænotypebestemmelse Myeloide afledte suppressorceller (MDSC) og berige Gr-1<sup> +</sup> Leukocytter fra mus milte. Denne metode anvender flowcytometri og AutoMACS Cell sortering for at berige for levedygtige Gr-1<sup> +</sup> Leukocytter før FACS-sortering af MDSC til anvendelse<em> In vivo</em> Og<em> In vitro</em> Assays.

Tags

Myeloid Derived Suppressor Cells (MDSC)Naive MicePancreatic Tumor-bearing MiceFlow CytometryAutomated Magnetic Activated Cell Sorting (AutoMACS)Heterogeneous PopulationImmature MacrophagesDendritic CellsGranulocytesLymphoid OrgansParasitic InfectionInflammationTraumatic StressGraft-versus-host DiseaseDiabetesCancerMac-1 (CD11b)Gr-1 (Ly6G And Ly6C)Tumor-bearing HostsAnti-tumor Immune ResponsesSubpopulations Of MDSCMolecular Mechanisms Of SuppressionGhansah GroupMurine Pancreatic Cancer ModelHomeostasisSuppressive FunctionLymphoid CompartmentsGr-1+ Leukocytes

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