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Chapters
- 00:05Title
- 03:11Generation of a Single-cell Suspension of Leukocytes from Spleens
- 05:09Cell Surface Staining/Immunophenotyping of MDSC using Flow Cytometry
- 09:01Magnetic Enrichment of Gr-1+ Leukocytes
- 12:06Post-sort Analysis of Gr-1-enriched Leukocytes
- 12:51AutoMACS Enrichment of Naive Gr-1+ Leukocytes for MDSC FACS Sorting
- 13:40Conclusion
Bu myeloid türevi bastırıcı hücreler (MDSC) işaretleyicidir ve Gr-1 zenginleştirmek için hızlı ve kapsamlı bir yöntemdir<sup> +</supFare dalak gelen> lökositlerin. Bu yöntem uygulanabilir Gr-1 için zenginleştirmek için sıralama akım sitometri ve AutoMACS Hücre kullanır<sup> +</supKullanım için MDSC sıralama FACS önce> lökositlerin<em> In vivo</em> Ve<em> In vitro</em> Deneyleri.
Tags
Myeloid Derived Suppressor Cells (MDSC)Naive MicePancreatic Tumor-bearing MiceFlow CytometryAutomated Magnetic Activated Cell Sorting (AutoMACS)Heterogeneous PopulationImmature MacrophagesDendritic CellsGranulocytesLymphoid OrgansParasitic InfectionInflammationTraumatic StressGraft-versus-host DiseaseDiabetesCancerMac-1 (CD11b)Gr-1 (Ly6G And Ly6C)Tumor-bearing HostsAnti-tumor Immune ResponsesSubpopulations Of MDSCMolecular Mechanisms Of SuppressionGhansah GroupMurine Pancreatic Cancer ModelHomeostasisSuppressive FunctionLymphoid CompartmentsGr-1+ Leukocytes
