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Chapters
- 00:05Title
- 03:11Generation of a Single-cell Suspension of Leukocytes from Spleens
- 05:09Cell Surface Staining/Immunophenotyping of MDSC using Flow Cytometry
- 09:01Magnetic Enrichment of Gr-1+ Leukocytes
- 12:06Post-sort Analysis of Gr-1-enriched Leukocytes
- 12:51AutoMACS Enrichment of Naive Gr-1+ Leukocytes for MDSC FACS Sorting
- 13:40Conclusion
Questo è un metodo rapido e completo di immunofenotipizzazione cellule derivate mieloidi soppressore (MDSC) e arricchimento Gr-1<sup> +</sup> Leucociti dal milza del mouse. Questo metodo utilizza la citometria a flusso e le celle a AutoMACS Ordinamento per arricchire di vitale Gr-1<sup> +</sup> Leucociti prima FACS smistamento di MDSC per uso<em> In vivo</em> E<em> In vitro</em> Saggi.
Tags
Myeloid Derived Suppressor Cells (MDSC)Naive MicePancreatic Tumor-bearing MiceFlow CytometryAutomated Magnetic Activated Cell Sorting (AutoMACS)Heterogeneous PopulationImmature MacrophagesDendritic CellsGranulocytesLymphoid OrgansParasitic InfectionInflammationTraumatic StressGraft-versus-host DiseaseDiabetesCancerMac-1 (CD11b)Gr-1 (Ly6G And Ly6C)Tumor-bearing HostsAnti-tumor Immune ResponsesSubpopulations Of MDSCMolecular Mechanisms Of SuppressionGhansah GroupMurine Pancreatic Cancer ModelHomeostasisSuppressive FunctionLymphoid CompartmentsGr-1+ Leukocytes
