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Colheita Murino macrófagos alveolares e Avaliação de ativação celular induzida por nanopartículas polianidrido
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles
DOI:

11:26 min

June 08, 2012

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Chapters

  • 00:05Title
  • 01:40Harvesting Alveolar Macrophages from the Mouse using Lung Lavage
  • 04:26Processing of Lung Lavage Harvest
  • 06:29Addition of Polyanhydride Nanoparticles and Control Treatments
  • 08:59Results from Flow-cytometric Analysis of Nanoparticle-induced Activation of Isolated Alveolar Macrophages
  • 10:46Conclusion

Summary

Automatic Translation

Descrevemos os protocolos para a colheita murinos macrófagos alveolares, que são residentes as células imunes inatas no pulmão, e examinando a sua ativação em resposta a co-cultura com nanopartículas polianidrido.

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