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Bioengineering
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收获小鼠肺泡巨噬细胞和评价聚酸酐纳米粒子诱导的细胞活化
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles
Please note that all translations are automatically generated.
Click here for the English version.
收获小鼠肺泡巨噬细胞和评价聚酸酐纳米粒子诱导的细胞活化
DOI:
10.3791/3883-v
•
11:26 min
•
June 08, 2012
•
Ana V. Chavez-Santoscoy
,
Lucas M. Huntimer
,
Amanda E. Ramer-Tait
,
Michael Wannemuehler
,
Balaji Narasimhan
1
Department of Chemical and Biological Engineering
,
Iowa State University
,
2
Department of Veterinary Microbiology and Preventive Medicine
,
Iowa State University
Chapters
00:05
Title
01:40
Harvesting Alveolar Macrophages from the Mouse using Lung Lavage
04:26
Processing of Lung Lavage Harvest
06:29
Addition of Polyanhydride Nanoparticles and Control Treatments
08:59
Results from Flow-cytometric Analysis of Nanoparticle-induced Activation of Isolated Alveolar Macrophages
10:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在此,我们描述了小鼠肺泡巨噬细胞,居住在肺先天免疫细胞,并检查其激活,共培养酸酐纳米收获的协议。
Tags
Murine Alveolar Macrophages
Cellular Activation
Polyanhydride Nanoparticles
Intranasal Vaccines
Respiratory Infectious Diseases
Sebacic Acid
1
6-bis(p-carboxyphenoxy)hexane
1
8-bis(p-carboxyphenoxy)-3
6-dioxaoctane
Bulk Erosion Kinetics
Surface Erosion Kinetics
Biomolecules Release
Adjuvant Activity
Vaccine Delivery Platform
Innate Immunity
Molecular Responses
Cellular Responses
Antigen Presenting Cells (APCs)
Dendritic Cells
Alveolar Macrophages (AM)
Lung Parenchyma
Microbial Pathogens
Cell Debris
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